23.8 What is Capillary Electrophoresis?
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This article is from the Chemistry FAQ, by Bruce Hamilton B.Hamilton@irl.cri.nz with numerous contributions by others.
23.8 What is Capillary Electrophoresis?
Capillary electrophoresis uses a small fused silica capillary that has been
coated with a hydrophilic or hydrophobic phase to separate biomolecules,
pharmaceuticals and small inorganic ions. A voltage is applied and the
analytes migrate and separate according to their charge under the specific
pH conditions, as also happens for electrophoresis. The capillary can also
be used for isoelectric focusing of proteins. The use of salt or vacuum
mobilisation is no longer required.
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