This article is from the Acoustics FAQ, by Andrew Silverman with numerous contributions by others.
The measurement method depends on the particular situation. There are standards for the measurement of the insulation of materials in the laboratory, and for a number of different field circumstances. Usually the procedures involve generating a loud sound of a specified type and monitoring the transmitted noise.
It is very useful to have a single number to characterize the insulation of a partition. Measurements are often conducted in third- octaves, and the reduction plotted on a graph. A reference curve is then fitted to the measurements using a specified procedure, and the value of this curve at 500 Hz is taken as the figure. There is a slight difference in procedure between the U.S. and ISO standards, but the methods are basically similar. The same is also true for impact noise transmission assessment, where a standard tapping machine is in use to hammer floors. Sound pressure levels in the room below are monitored.
 
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